Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing

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Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing

BACKGROUND The recent discovery of residing tendon stem/progenitor cells has triggered a growing interest in stem cells as a useful tool in tendon repair. Our knowledge of their involvement in naturally healing tendons is, however, sparse. The aim of this study was to identify and determine stem/progenitor cells in relation to different healing phases and regions in a rat model of Achilles tend...

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Rat Achilles tendon healing: mechanical loading and gene expression.

Injured tendons require mechanical tension for optimal healing, but it is unclear which genes are upregulated and responsible for this effect. We unloaded one Achilles tendon in rats by Botox injections in the calf muscles. The tendon was then transected and left to heal. We studied mechanical properties of the tendon calluses, as well as mRNA expression, and compared them with loaded controls....

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Ultrastructural determinants of murine achilles tendon strength during healing.

The mechanisms by which tendon strength is established during growth and development and restored following injury are not completely understood and are likely to be complex, multifactorial processes. Several studies examining the relationship between mechanical behavior and ultrastructural characteristics of tendons and ligaments during growth and maturation suggest that collagen fibril diamet...

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Microtrauma stimulates rat Achilles tendon healing via an early gene expression pattern similar to mechanical loading.

Mechanical loading increases the strength of healing tendons, but also induces small localized bleedings. Therefore, it is unclear if increased strength after loading is a response to mechanotransduction or microtrauma. We have previously found only five genes to be up-regulated 15 min after a single loading episode, of them four were transcription factors. These genes are followed by hundreds ...

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Achilles Tendon Healing in Inos Knockout Mice

Nitric oxide (NO), a diatomic free radical, regulates the functions of many mammalian cells. We have previously reported that nitric oxide synthases (iNOS, eNOS and bNOS) are induced during rat tendon healing and inhibition of nitric oxide synthases (NOS) inhibited rat tendon healing [1]. The aim of this study was to evaluate the effects of deleting the iNOS gene on Achilles tendon healing in m...

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ژورنال

عنوان ژورنال: BMC Musculoskeletal Disorders

سال: 2015

ISSN: 1471-2474

DOI: 10.1186/s12891-015-0658-3